Protein derived from tick saliva proves effective in the treatment of equine skin cancer

Experiments were conducted by scientists affiliated with the Centre of Excellence in New Target Discovery, a research centre supported by FAPESP, involving five animals with spontaneous skin tumours.

A protein derived from the saliva of the tick Amblyomma sculptum has been successfully used by researchers at the Butantan Institute in São Paulo, Brazil, to treat skin cancer (melanoma) in horses.

The results of the study are described in the journal Scientific Reports.

The principal investigator for the project is Ana Marisa Chudzinski-Tavassi, who leads the Centre of Excellence in New Target Discovery (CENTD), an Engineering Research Centre (ERC) established at the Butantan Institute by São Paulo Research Foundation – FAPESP and GlaxoSmithKline.
CENTD’s mission is to discover and validate molecular targets to treat inflammatory diseases for the purpose of developing new drugs.

The protein is Amblyomin-X, which has been studied at the Butantan Institute for more than ten years and evidences significant anti-tumour potential in the laboratory and in vivo and has already been approved in preclinical toxicity testing.

The researchers used “omics” tools (genomics, transcriptomics, proteomics and metabolomics) to analyse the signalling pathways and proteins affected by the treatment of horse melanoma with Amblyomin-X.

Roger Chammas, a researcher at the São Paulo State Cancer Institute (ICESP), collaborated with CENTD scientists on the project.

Immune mechanisms

Equine melanomas are spontaneous tumours. Unlike cutaneous melanomas in humans, in horses, they are encapsulated and locally confined, rarely giving rise to metastasis. However, in both humans and horses, these tumours are potentially immunogenic, i.e., capable of producing an immune response, and can serve as a model for the investigation of the immune mechanisms involved in tumour regression induced by therapeutic molecules.

In the first phase of the study, spontaneous tumours in five horses were treated for 30 days with intratumoural injections of Amblyomin-X. The treatment took place at the Butantan Institute’s farm in Araçariguama, state of São Paulo. The animals were monitored throughout the period by means of clinical examinations, blood work, and biochemical laboratory tests.

The results were promising. Control tumours (not treated with the compound) maintained their shape and grew in size, whereas tumours treated with Amblyomin-X shrank and, in some cases, even reached remission up to two months after the treatment ended.

None of the five treated animals developed any adverse reactions. At the end of the treatment, the tumours were surgically removed for histopathological analysis. The researchers found no trace of tumoural characteristics.

The second phase of the study consisted of experiments in transcriptomics (analysing messenger RNA expression) and interactomics (analysing the interaction between tumour cell proteins and Amblyomin-X) to see how signalling pathways were modulated by the treatment and, especially, to obtain molecular confirmation of previous findings from in vitro and in vivo experiments conducted by Chudzinski-Tavassi and her team. The results published by the group between 2010 and 2017 suggested that the antitumour action of Amblyomin-X occurred via the modulation of the endoplasmic reticulum and mitochondrial stress and apoptotic and proteasomic pathways, among others.

The transcriptome study was expected to confirm activation of these pathways and show whether others were affected by the treatment. The researchers also deployed next-generation sequencing and analysis based on bioinformatics and systems biology to map the initial mechanisms of the response triggered by Amblyomin-X, which culminated in tumour regression.

Among the findings of the transcriptomic analysis was the discovery that the innate immune system’s rapid response (six hours after the injections) involved the modulation of four different pathways: TLR (toll-like receptor), RIG-I (viral invasion sensors), OAS (2’,5’- oligoadenylate synthetase and RNase L) and oncostatin-M (corresponding to the interleukin-6 family inflammation pathway). These findings described the first steps in the activation of a defence response that culminated in tumour regression, pointing to potential candidate targets for adjuvant therapies against tumours.

Journal Reference:
Lichtenstein, F., Iqbal, A., de Lima Will, S.E.A. et al. Modulation of stress and immune response by Amblyomin-X results in tumor cell death in a horse melanoma model. Sci Rep 10, 6388 (2020). https://doi.org/10.1038/s41598-020-63275-2

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